Fig. 3: Differential expression of sialyltransferase gene and suppression of ST6Gal-1 expression by AOS in prostate cancer. | Cell Death & Disease

Fig. 3: Differential expression of sialyltransferase gene and suppression of ST6Gal-1 expression by AOS in prostate cancer.

From: RETRACTED ARTICLE: Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway

Fig. 3

a–c mRNA levels of the sialyltransferase (ST) gene family without or with 500 µg/ml AOS administration in DU145 cells determined by real-time quantitative PCR (qPCR) and normalized for GAPDH. d Relative ≥2-fold intensity ratios of the ST genes observed. e–i Determination of ST6Gal-1 expression in both DU145 and PC-3 cells after 100 and 500 µg/ml AOS treatment by qPCR (e, f), western blot (g, h), and lectin blot (i). Cells following ST6Gal-1 overexpression transfection were detected by lectin blot (i). Data represent the mean ± SD of the expression levels of three independent experiments (*P < 0.05)

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