Fig. 7: GSNO induces NCI-H1299 apoptosis via FOXO1 activation. | Cell Death & Disease

Fig. 7: GSNO induces NCI-H1299 apoptosis via FOXO1 activation.

From: S-nitrosylation of the Peroxiredoxin-2 promotes S-nitrosoglutathione-mediated lung cancer cells apoptosis via AMPK-SIRT1 pathway

Fig. 7

a, b The interaction between SIRT1 and FOXO1 (*P < 0.05, **P < 0.01, using unpaired two-tailed Student’s t-test). c Detection of FOXO1 acetylation by immunoprecipitation (**P < 0.01, using unpaired two-tailed Student’s t-test). d FOXO1 expression in the cytoplasm and nucleus after GSNO treatment (**P < 0.01, ***P < 0.001, using one-way ANOVA followed by Bonferroni’s multiple comparisons test). e The mRNA quantification of Bim and Puma (*P < 0.05, using one-way ANOVA followed by Bonferroni’s multiple comparisons test). f Immunoprecipitation detection of FOXO1 acetylation with GSNO treatment or SIRT1 knockdown (*P < 0.05, ***P < 0.001, using unpaired two-tailed Student’s t-test). g, h The expression of FOXO1 and phosphorylated AKT with GSNO treatment or SIRT1 knockdown (*P < 0.05, **P < 0.01, using unpaired two-tailed Student’s t-test). i Schematic representation of the mechanism by which GSNO promotes apoptosis in lung cancer cells. The data are expressed as the mean ± SD of three independent experiments

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