Fig. 4: Flow cytometry analysis of EP on apoptosis and cell cycle progression. | Cell Death & Disease

Fig. 4: Flow cytometry analysis of EP on apoptosis and cell cycle progression.

From: Blockade of HMGB1 signaling pathway by ethyl pyruvate inhibits tumor growth in diffuse large B-cell lymphoma

Fig. 4

ABC-DLBCL Su-2 cells were cultured with or without indicated concentrations of EP for 24 h. a, c Apoptotic cell death was measured by flow cytometry after staining with annexin V-FITC and PI. b, d Cell cycle distribution analysis by quantitation of DNA content. Cells were stained with PI and DNA content was analyzed by flow cytometry. e, f Cell cycle analysis with Ki67 and PI. Permeabilized cells were stained with Ki67-FITC and PI and DNA content was analyzed by flow cytometry. The percentages of cells within each cell cycle phase are depicted by density plots. c, d, f Data shown (mean ± SD) are from three independent experiments. The significant difference between EP-treated and control was analyzed by the student t-test

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