Fig. 6: EP-induced interaction between P27 with cyclin A and CDK2. | Cell Death & Disease

Fig. 6: EP-induced interaction between P27 with cyclin A and CDK2.

From: Blockade of HMGB1 signaling pathway by ethyl pyruvate inhibits tumor growth in diffuse large B-cell lymphoma

Fig. 6

ABC-DLBCL cells Ly-3 and Su-2 (a) and GCB-DLBCL cells Su-8 and Ly-7 (b) were treated with 2-mM EP for 24 h. a, b Protein complex was immuno-precipitated with a rabbit anti-p27 antibody and blots were probed with either a mouse anti-CDK2, a mouse anti-cyclin A, or a rabbit anti-p27 antibodies, respectively. c Immunofluorescent determination of colocalization of p27 and CDK2 in Su-2 and Ly-7 cells. After treatment, cells on slides were costained with mouse anti-CDK2 (red)/rabbit anti-p27 (green) antibodies. Secondary antibodies for costaining were FITC-conjugated goat anti-rabbit IgG and Rhodamine-conjugated goat anti-mouse IgG. White arrows indicate cytoplasm and red arrows indicate colocalization of p27 and CDK2. Colocalization coefficient (Rr) was analyzed by the intensive correlation analysis program using WCIF ImageJ software. Scale bar: 10 µm

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