Fig. 8: JQ1 induces ferroptosis via the regulation of genes associated with ferroptosis. | Cell Death & Disease

Fig. 8: JQ1 induces ferroptosis via the regulation of genes associated with ferroptosis.

From: Ferritinophagy is required for the induction of ferroptosis by the bromodomain protein BRD4 inhibitor (+)-JQ1 in cancer cells

Fig. 8

a, b Kyoto Encyclopedia of Genes and Genomes (a) term enrichment and Gene Ontology (b) pathway analysis data were analyzed using the DAVID Functional Annotation Bioinformatics Microarray Analysis tool (https://david.ncifcrf.gov/). The vertical axis represents the biological process or pathway category, and the horizontal axis represents −log10 (P value) of these significant biological processes or pathways. c The expression of the ferroptosis-associated genes GPX4, SLC7A11, and SLC3A2 was downregulated with BRD4 knockdown. The gene expression values were normalized to GAPDH expression. The expression values in the Scramble groups were normalized to 1. The data represent the results of at least three independent experiments. d The expression of BRD4 was positively related to the expression of GPX4 (correlation coefficient (R) = 0.125), SLC7A11 (R = 0.183), and SLC3A2 (R = 0.185). The data were analyzed through the calculation of pairwise Pearson correlation coefficients. e The expression of the ferroptosis-associated genes GPX4, SLC7A11, and SLC3A2 was downregulated under JQ1 (10 μM, 24 h) treatment. The gene expression values were normalized to GAPDH expression. An equal volume of DMSO was used as the control. The expression value of the DMSO group was normalized to 1. *P < 0.05, **P < 0.01, ***P < 0.001. The data represent the results of at least three independent experiments

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