Fig. 1: Heterogeneity in human umbilical cord derived MSCs.

a Process for preparation of MSCs for single cell sequencing, including MSCs isolation from the umbilical cord, in vitro expansion, pretreatment approach, single cell capture, and RNA sequencing. b Sample information for RNA sequencing. c PCA analysis performed on UC-MSCs from the two different donors and three passages. Each plot represents an independent analysis. Cells are grouped into four distinct clusters. d Key modules of MSC heatmap revealed by WGCNA, with the significance of each module in each subcluster. Each cell contains the p value. Data are representative from huc2_p0. e Heatmap of module preservation scores among different datasets. Module preservation scores are represented by the Z-summary score. f Expression profile of featured genes in four subclusters are shown as box plot. Data are representative from huc2_p0