Fig. 2: Inflammatory cytokines stimulated MSCs subpopulations also exhibit heterogeneity in the featured genes.

a Sample information for RNA sequencing. Cells are pretreated with IFNγ and TNFα (10 ng/mL) for 12 h. b Unsupervised hierarchical clustering of MSCs with IFNγ and TNFα pretreatment. Data are representative from huc2_sti_p2. c WGCNA reveals the key modules of the heatmap of MSCs stimulated by inflammatory cytokines, IFNγ and TNFα, showing the significance of each module in each subcluster. Each cell contains the P value. Data are representative from huc2_sti_p2. d Heatmap of module preservation score among different datasets. Module preservation scores are represented by the Z-summary score. e, f PCA analysis shows the gene clustering in huc2_sti_p2 as representative. Expression profile of featured genes (e) and immunomodulatory genes (f) is analyzed. Red to blue shows the level from high to low