Fig. 4: Knockdown of EZH2 enhanced CP-induced mTEC apoptosis. | Cell Death & Disease

Fig. 4: Knockdown of EZH2 enhanced CP-induced mTEC apoptosis.

From: 3-deazaneplanocin A protects against cisplatin-induced renal tubular cell apoptosis and acute kidney injury by restoration of E-cadherin expression

Fig. 4: Knockdown of EZH2 enhanced CP-induced mTEC apoptosis.

mTECs were transfected with siRNA targeting EZH2 or scrambled siRNA (a–g) and then exposed to cisplatin (20 μg/ml) for an additional 24 h (c–g). Cell lysates were prepared and subjected to immunoblot analysis with antibodies against cas3, EZH2, H3K27me3, tubulin, and β-actin (a, c). The levels of cleaved-cas3 (d), EZH2 (b, d), or H3K27me3 (d) were quantified by densitometry and normalized with tubulin or β-actin. Cell viability was detected by CCK-8 assay (f). Cell apoptosis was detected by DAPI staining and the incidence of apoptosis was analyzed by enumerating nuclear of deep dyeing cells with condensed chromatin (e), and expressed as the percentage of apoptotic cells (g). Data represent the mean ± SEM of at least three experiments. Bars with different letters (a–c) for each molecule are significantly different from one another (P < 0.05)

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