Fig. 4: STAT3 mediated c-Myc expression is required for IL-11 induced osteoclastogenesis. | Cell Death & Disease

Fig. 4: STAT3 mediated c-Myc expression is required for IL-11 induced osteoclastogenesis.

From: IL-11 is essential in promoting osteolysis in breast cancer bone metastasis via RANKL-independent activation of osteoclastogenesis

Fig. 4

a–c Western blot analysis of p-JAK1, JAK1, p-STAT3, STAT3, c-Myc, c-Fos, NFATc1 and GAPDH in wild type (a), STAT3−/− (b) and c-Myc−/− (c) mouse BMMs induced with M-CSF + RANKL, M-CSF + IL-11 or M-CSF + BoM1833 CM. d Representative TRAP stain images of wild type, STAT3−/− and c-Myc−/− mouse BMMs induced with M-CSF + RANKL, M-CSF + IL-11 or M-CSF + BoM1833 CM. Bar represents 200 μm. e Quantification of relative TRAP activity and osteoclast number per well in (d). The data in the figures represent the averages ± SD. Significant differences are indicated as *p < 0.05 or **p < 0.01 paired using Student’s t test unless otherwise specified

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