Fig. 6: Blocking of STAT3 phosphorylation by AG-490 reduces osteolysis and tumor growth in bone metastatic breast cancer.

a BoM-1833^Luc cells were injected intrafemorally in female athymic mice followed by i.v. injection of AG-490 every two days for 4 weeks (n = 8 for each group). b schematic representation of the experimental design. c Tumor incidence in (a). d Kaplan–Meier survival analysis representing the overall survival of BoM-1833 cell injected mice treated with normal saline (control) or AG-490, **p < 0.01, log-rank test. e Representative μCT of longitudinal section femurs (left) and quantification of osteolytic lesion volume (right). f Representative μCT images of reconstructed distal femurs. Quantification of trabecular number (Tb. N), trabecular separation (Tb. Sp), trabecular thickness (Tb.Th) and cortical thickness (Ct.Th). g Representative in vivo BLI images and quantification of normalized photon flux. h Representative TRAP stain images of distal femurs and quantification of osteoclast surface/bone surface. Bar represents 200 μm. i Representative immunohistochemical (IHC) stain images of p-STAT3 of distal femurs and quantification of IHC scores. Bar represents 200 μm (left) and 50 μm (right). The data in the figures represent the averages ± SD. Significant differences are indicated as *p < 0.05 or **p < 0.01 paired using Student’s t test unless otherwise specified