Fig. 3: NCKAP1 expression in a normal liver cell line and hepatocellular carcinoma (HCC) cell lines.

a Western blotting results show that L02, SMMC-7721, and SK-Hep-1 cells exhibited low expression compared to that of Hep-3B and Huh-7 cells. GAPDH was used as a control. b Quantitative real-time PCR (qPCR) results confirmed the high expression of NCKAP1 in Hep-3B and Huh-7 cells. c Overexpression of NCKAP1 (OE) in a transfected SMMC-7721 cell line verified by western blotting and qPCR compared to that of cells transfected with the control vector (Vec). GAPDH was used as a control. d Overexpression of NCKAP1 in a transfected SK-Hep-1 cell line verified by western blotting and qPCR. GAPDH was used as a control