Fig. 7: WASF1, Rb1, and p53 expression in hepatocellular carcinoma (HCC) and breast cancer cell lines.

a Western blotting results show that HCC cell lines (7721, SK, 3B, and HUH7) exhibited fairly low expression of WASF1 compared to that of breast cancer cell lines (MC-7 and MDA-MB-231). GAPDH was used as a control. b NCKAP1-overexpressing (OE) HCC cell lines (SMMC-7721 and SK-Hep-1) showed upregulated expression of Rb1, p53, cyclin D1, p27, cdk2, p18, cdk4, and cdk6 compared to that of cells transfected with the vector control (Vec), as detected by western blotting. GAPDH was used as a control