Fig. 4: The effects of oridonin on myocardial autophagy.
From: Oridonin protects against cardiac hypertrophy by promoting P21-related autophagy
a, b Immunoblotting and quantification analyses of the protein levels of LC3-I/II, P62, Atg5-12, Atg7, and Beclin1 in oridonin- and vehicle-treated mice after 1 week of AB surgery (n = 6). c–e Detection of LC3 expression by immunofluorescence staining in the indicated mice after 1 week of AB (scale bar: 20 μm, n = 6), and in H9C2 cardiomyocytes (scale bar: 20 μm, n = 6). Arrows indicate LC3-II dots. *P < 0.05 versus vehicle-sham. #P < 0.05 versus vehicle-AB (left panel). *P < 0.05 versus vehicle-PBS. #P < 0.05 versus vehicle-AngII (right panel). f Representative transmission electron microscope images of cardiomyocytes 1 week post AB surgery. Arrows indicate autophagosomes and autolysosomes containing electron-dense contents. Scale bars: 500 nm (up) and 200 nm (down). g, h Relative LC3-II/GAPDH levels after Baf A1 intraperitoneal injection in mice (n = 6, †P < 0.05)
