Fig. 6: The effects of oridonin were blocked by P21-deficiency or autophagy decay in vivo.

a, b Representative Western blot analyses of LC3-I/II and P62 (n = 6). c, d Representative fluorescence images of cardiomyocytes transfected with Cherry-red fluorescent protein (RFP)-green fluorescent protein (GFP)-LC3 adenovirus 2 h after Ang II treatment. Autophagosome (yellow dots) and autolysosome (red dots) numbers in cardiomyocytes after Ang II (2 h)/PBS treatment with or without BafA1 (100 nM) were calculated. (scale bar: 20 μm, n = 30 cells per group). e Representative images of a-actinin staining of the cardiomyocytes. P21 adenovirus-infected cells were stimulated with Ang II for 2 h, and then treated with oridonin with or without 3-MA (5 mM) as indicated. (scale bar: 20 μm). f Statistical results of cell area by measuring random cells (n = 30 cells). *P < 0.05 versus PBS. #P < 0.05 versus Ang II. n.s. not significant