Fig. 8: Manipulation of P21 levels in mice affects the antihypertrophic effect of oridonin.
From: Oridonin protects against cardiac hypertrophy by promoting P21-related autophagy
a Histological analysis of whole hearts and heart sections stained with H&E (the upper and middle panel, scale bar: 1000 μm and 20 μm, n = 6). Representative immunohistochemical analysis of 4-HNE (the lower panel, scale bar: 20 μm, n = 6) in mouse hearts from the indicated groups 4 weeks after AB or sham surgery. b Statistical results for the cross-sectional areas and 4-HNE fold change in mouse hearts (n = 100 cells). c Statistical results of HW/BW and HW/TL (n = 6). d, e Summarized echocardiographic measurements of diastolic wall thickness [interventricular septum (IVS) + posterior wall (PW)] and fraction shortening (FS). f Real-time PCR analyses of the transcriptional levels of ANP, BNP, and β-MHC (n = 6). g, h Representative Western blot analyses of P-AKT, T-AKT, P-AMPK, T-AMPK and LC3-I/II (n = 6). Data are represented as the means ± SD. *P < 0.05 versus vehicle-sham. #P < 0.05 versus vehicle-AB. §P < 0.05 versus AB-oridonin. n.s. not significant
