Fig. 5: miR-30 sponge-induced uPAR-ITGB3 activation resulted in increases of RAC1 and CDC42 activities, and downregulation of synaptopodin. | Cell Death & Disease

Fig. 5: miR-30 sponge-induced uPAR-ITGB3 activation resulted in increases of RAC1 and CDC42 activities, and downregulation of synaptopodin.

From: MiR-30 family prevents uPAR-ITGB3 signaling activation through calcineurin-NFATC pathway to protect podocytes

Fig. 5: miR-30 sponge-induced uPAR-ITGB3 activation resulted in increases of RAC1 and CDC42 activities, and downregulation of synaptopodin.

a Pull-down assay of active Rho GTPases showed that the activities of both RAC1 and CDC42 were increased by miR-30 sponge, but the increases were blocked by uPAR siRNA and cyclo-RGDfK. Quantifications of the results were shown based on three independent experiments: One-way ANOVA, *P < 0.05 verses mock group, or vs. the sponge + vehicle group, respectively. b Western blotting showing that both uPAR siRNA and cyclo-RGDfK reversed the miR-30 sponge-induced synaptopodin downregulation. Quantifications of the results based on three independent experiments: Two-way ANOVA, *P < 0.05. All values were expressed as the means ± SD

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