Fig. 6: Cul4a activates scl-α and gata1 transcription by an epigenetic mechanism.
From: Cul4a promotes zebrafish primitive erythropoiesis via upregulating scl and gata1 expression
a, b E-qChIP assays of wild-type zebrafish at 24 hpf with antibodies against Cul4a (a) and H3K4me3 (b). The presence of scl promoter sequences in the input DNA and antibody-bound chromatin segments was analyzed by qPCR and agarose gel electrophoresis. c, d E-qChIP assays of scl at 24 hpf embryos injected with CoMO or cul4a-MO. e, f The presence of gata1 promoter sequences in the input DNA and antibody-bound chromatin segments was analyzed by qPCR and agarose gel electrophoresis. g, h E-qChIP assays of gata1 at 24 hpf embryos injected with CoMO or cul4a-MO. Experiments were performed in triplicate. ***p < 0.001, **p < 0.01
