Fig. 7: Rescued expression of Rack1 restores the chemoresistance.

a Western blotting analysis of the expression level of Flag and Rack1 in cell lysates from wild-type, control, Rack1-silenced cells, and Rack1^WT- and Rack1^Y246F-rescued cells. β-Actin was used as a loading control. b Rescued expression of Rack1^WT, but not Rack1^Y246F mutant, recovered cell viability in response to EPI. Wild-type, control, and Rack1-rescued cells were seeded in 96-well plates, then different concentrations of EPI were added into the cells, and drug sensitivity assay was performed by CCK8-based assay as described above. Values were expressed as mean ± SD from three independent experiment. c Rescued expression of Rack1^WT rather than Rack1^Y246F decreased the accumulation of Rh123 within cells as measured by flow cytometry. d Rescued expression of Rack1^WT significantly enhances the efflux rate of Rh123 compared with control or Rack1^Y246F-rescued cells. Data were presented as mean ± SD, statistical analysis was performed using two-way ANOVA, ***P < 0.001, ****P < 0.0001 vs. control