Fig. 6: Chloroquine ameliorates CRH-induced colonic damage and Paneth cell metaplasia in IBD mice. | Cell Death & Disease

Fig. 6: Chloroquine ameliorates CRH-induced colonic damage and Paneth cell metaplasia in IBD mice.

From: Intestinal autophagy links psychosocial stress with gut microbiota to promote inflammatory bowel disease

Fig. 6

DSS (3%) was given to C57BL/6 mice for 6 days while water was given to the control group. For certain groups, CRH (50 μg/kg body weight) and/or chloroquine (60 mg/kg body weight) was intraperitoneally given from day 1 to day 6 and saline was injected as vehicle. a–c Body weight, the presence of occult or gross blood per rectum, stool consistency, and colon length were determined by two investigators blinded to the treatment groups. Compared to the DSS + Vehicle group, the mice in the DSS + CRH group exhibited significant deterioration in body weight loss, bloody stool score, and colon length, while the administration of chloroquine largely attenuated the aggravated effect of CRH (n = 8 per group). **P < 0.01 vs. the control group, ##P < 0.01 vs. the DSS + Vehicle group, $$P < 0.01 vs. the DSS + CRH group. d–h The left edge of the left colon was isolated and the levels of TNF-α, IL-18, IL-7, MPO, and the MPO activity in the left colon were analyzed by ELISA and O-dianisidine method. Compared to the DSS + Vehicle group, the mice in the DSS + CRH group showed aggravated colonic inflammation, while the administration of chloroquine largely attenuated the effect of CRH (n = 6 per group). *P < 0.05, **P < 0.01. i The left edge of the left colon was isolated and fixed and H&E staining was used for the detection of histological score. Compared to the DSS + Vehicle group, the mice in the DSS + CRH group showed aggravated inflammatory infiltration in the left colon, while the administration of chloroquine largely attenuated the effect of CRH (n = 8 per group). *P < 0.05, **P < 0.01. j The left edge of the colon was separated and fixed and immunohistochemical staining was used for the detection of Paneth cell. IOD values were analyzed. Compared to the DSS + Vehicle group, the mice in the DSS + CRH group showed enhancement of Paneth cell metaplasia in the epithelium of the left colon, while the administration of chloroquine largely attenuated the effect of CRH (n = 6 per group). *P < 0.05, **P < 0.01

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