Fig. 7: In vivo activity of BTG2/TIS21 gene in the TIS21-knockout mice and human breast cancers.

a To analyze in vivo regulation of Twist1 expression by BTG2/TIS21 gene, five organs were extracted from each TIS21-KO (six heads) and the WT (six heads) female mice, and then subjected to immunoblot analyses with 40 µg/lane in each sample. When the ratios of Twist1/α-tubulin expression were examined by Image J software, the level was significantly higher in the TIS21-KO mice than that in the WT (n = 30 organs in each group). b Relapse-free survival vs. Twist1 expression was analyzed from open data in the HER2+ and Basal-type breast cancers. Higher expression of Twist1 significantly decreased the relapse free survival of HER2+ (p < 0.05) and Basal-type (p < 0.01) breast cancers. c Relapse-free survival vs. BTG2 expression was analyzed from open data in the HER2+ and Basal-type breast cancers. Higher expression of BTG2 significantly increased the relapse free survival of the HER2+ (p < 0.001) and Basal-type (p < 0.001) breast cancer patients