Fig. 1: Original and manipulated expression of SASH1 in astrocytes and C6 glioma cells.

A The original SASH1 protein expression in C6 rat glioma cells and cultured rat astrocytes. β-Actin was used as the internal control. a Representative Western blots. b The statistical results. Astrocytes vs. C6 cells, n = 3, P = 0.0113. B The mRNA and protein levels of SASH1 in astrocytes after treatment with SASH1 siRNA. a The statistical results of qRT-PCR analysis. b Representative Western blots (left) and the statistical results (right). SASH1 siRNA (siSASH1) vs. control siRNA (siControl), n = 3, P = 0.0024 (mRNA), P = 0.0313 (protein). C The results of the EdU assay on cultured astrocytes. a Representative images of the EdU assay results in astrocytes after siControl and siSASH1 treatment. Panel b The statistical results. siSASH1 vs. siControl, n = 4, P = 0.0327. D The mRNA and protein levels of SASH1 in C6 cells after Adv4-SASH1 application. a The statistical results of qRT-PCR analysis. b Representative Western blots (left) and the statistical results (right). Adv4-SASH1 vs. control Adv4 vector (Adv4-NC), n = 3, P = 0.0032 (mRNA), P = 0.0268 (protein). E The results of the EdU assay on C6 glioma cells. a Representative images of the EdU assay results in C6 cells after Adv4-NC and Adv4-SASH1 treatment. b The statistical results. Adv4-SASH1 vs. Adv4-NC, n = 4, P = 0.006