Fig. 1: ISO acutely causes cardiomyocyte necrosis mainly targeting the sub-endocardial apex. | Cell Death & Disease

Fig. 1: ISO acutely causes cardiomyocyte necrosis mainly targeting the sub-endocardial apex.

From: c-kit Haploinsufficiency impairs adult cardiac stem cell growth, myogenicity and myocardial regeneration

Fig. 1

a The fraction of necrotic cardiomyocytes (CM) was significantly increased in a dose-dependent manner at 1 day after ISO (200 and 400 mg/Kg) with higher frequency in the Apex sub-endocardium. n = 7 per group; *p < 0.05 vs. saline; #p < 0.05 vs. Epi; †p < 0.05 vs. Mid; ‡p < 0.05 vs. 200 mg/Kg (one-way ANOVA analysis with Tukey’s multiple comparison test). b Representative images of necrotic CMs labelled in vivo with anti-myosin antibody (green) showing higher damage in the sub-endocardium when compared with myocardium and epicardium. c, d Representative images of necrotic CMs labelled in vivo with anti-myosin antibody (green) showing ISO dose-dependent higher damage in the apex when compared with mid and base in LV regions 1 day after ISO 200 (c) or 400 (d) mg/Kg, respectively. e Representative image of a non-necrotic anti-myosin antibody (green)-labelled normal cardiomyocyte. f Plasma cTnI was significantly elevated in a dose-dependent manner at 1 day after ISO. hs-TnT = high-sensitive cardiac Troponin T. n = 7 per group; *P < 0.05 vs. Saline; #p < 0.05 vs. 200 mg/Kg (one-way ANOVA analysis with Tukey’s multiple comparison test). Scale bars = 50 µm except for b left panel = 300 µm. All data are mean ± SD

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