Fig. 2: Sprouty1 (SPRY1) expression during adipogenesis. | Cell Death & Disease

Fig. 2: Sprouty1 (SPRY1) expression during adipogenesis.

From: Sprouty1 is a weight-loss target gene in human adipose stem/progenitor cells that is mandatory for the initiation of adipogenesis

Fig. 2

a Western blot analysis of sprouty1 and perilipin levels in ASCs. Left panel: Adipogenesis was induced on d0. β-actin served as input control. Molecular masses are given in kDa. Right panel: Densitometric quantification of the Western blot. Values are expressed as mean ± SEM, n = 3. Statistical comparison to d0 was achieved using one-way ANOVA and Dunnett´s Multiple Comparison test. b mRNA expression analysis of SPRY1.1, PPAR γ2, adiponectin (ADIPOQ) and FABP4 using RT-qPCR in differentiating ASCs. Values are expressed as mean ± SEM, n = 3. Statistical comparison to d0 was achieved using ANOVA. c Oil Red O staining of ASCs is shown prior the induction of differentiation (d0), on d9 and d14 of adipogenesis. Magnification ×10. Scale bar 100 µm. In a–c representative results from one out of four donors are shown

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