Fig. 3: Knock-down of Sprouty1 in ASCs inhibits adipogenic differentiation. | Cell Death & Disease

Fig. 3: Knock-down of Sprouty1 in ASCs inhibits adipogenic differentiation.

From: Sprouty1 is a weight-loss target gene in human adipose stem/progenitor cells that is mandatory for the initiation of adipogenesis

Fig. 3

a Western blot analysis of Sprouty1 and perilipin protein levels in Sprouty1 KD ASCs. A non-targeting control shRNA (Ctrl.) and two Sprouty1-targeting shRNAs (shRNA #1 and #2) were employed. Adipogenesis was induced on d0. β-actin served as input control. Molecular masses are given in kDa. b Densitometry of the Western blot shown in a) (corresponding time-point d9). Values are expressed as mean ± SEM, n = 3. Statistical comparison to the appropriate control was achieved using the two-tailed paired t-test. c Oil Red O staining of differentiated ASCs on d14. Representative example of n = 3 independent experiments (i.e., donors). Magnification ×10. Scale bar 100 µm. d Photometric quantification of Oil Red O staining (d14) from n = 3 different donors. Values are expressed as mean ± SEM. Statistical comparison was done using the two-tailed unpaired t-test

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