Fig. 1: Released CD99 affects cell growth in EWS recipient cells.

a Representative western blotting showing CD99 secreted in the supernatants of eight EWS cell lines; CD99 protein expression in the corresponding EWS cells is shown for comparison. GAPDH was used as a loading control. b Evaluation of cell growth in serum-free condition in EWS cells receiving CD99pos- or CD99neg EXOs isolated from the TC-71 (purple bars) and IOR/CAR (green bars) experimental models. Columns show the mean values of at least two independent biological experiments with two replicates/each, and the bars represent the SE (*p < 0.05, **p < 0.01, Student's t-test, n = 2–4)