Fig. 6: Analysis of MCM7 function in liver CSC and MCM7 “rescue” assays for ATO treatment.
From: Arsenic trioxide inhibits liver cancer stem cells and metastasis by targeting SRF/MCM7 complex
a The flow cytometry analysis of the CD13+ percentage in psicoR-shMCM7 stable cells. b shows a fluorescence image of the tumorspheres (n = 3) from psicoR-shMCM7-GFP cells and scramble cells. Bars, 200 μm. c Gross observation of tumor-bearing NOD/SCID mice and peeled tumors derived from HCCLM3-psicoR-shMCM7-GFP cells and scramble cells at two dilutions (left). Right shows the comparison of tumor weight between the shMCM7 group and control group. d The dilutions and mouse numbers. e Representative examples (left) and quantitative analysis (right) of the IHC staining of MCM7 in the tumors derived from HCCLM3-psicoR-shMCM7 cells and scramble cells. f–h Rescue assay of CSCs by the ectopic expression of MCM7. Western blot analysis of MCM7 expression in transfected HCC cells treated with ATO or not (f). Tumorsphere formation was observed (g) and quantified (h, n = 3). Bars, 1000 μm. P value was determined by two-sided Student’s t-test
