Fig. 1: Expression profiles of miRNAs in GC cell lines and GC tissues.
a The differentially expressed miRNAs in 387 GC samples and 41 adjacent gastric mucosal tissues are visualised in a volcano plot. The red and green colours indicate high and low expression (|log2(fold change) | > 1, FDR < 0.05), respectively. b Heatmap of six polycistronic miRNAs derived from MIR17HG in the TCGA data set. c Heatmap of six polycistronic miRNAs derived from MIR17HG in the GEO verification data set (GSE93415 and GSE23739). b, c the columns represent the clinical samples, while the rows show the relative expression of individual miRNAs. The red and blue colours indicate high and low expression, respectively. d qRT-PCR analysis of the expression of six polycistronic miRNAs derived from MIR17HG in GC cell lines (AGS, MKN45 and SGC7901) and a normal gastric epithelial cell line (GES1). e qRT-PCR analysis of the expression of six polycistronic miRNAs derived from MIR17HG in 10 pairs of cancerous and adjacent tissues harvested from patients with GC. d, e U6 snRNA served as the internal control. N = 3 independent experiments performed in triplicate. *P < 0.05, as demonstrated by paired Student’s t test. The data are presented as the means ± standard deviations (SDs). f Receiver operating characteristic (ROC) curve of MIR17HG, miR-18a and miR-19a among GC patients
