Fig. 2: Cisplatin resistance mediated by cell growth in three-dimensional (3D) culture is independent of p53 and autophagy activity.

a, b Immunoblot for p53 and GAPDH in total lysates from MCF-7 cells deficient for p53 (shp53) and shRNA scramble control (ctrl) treated with cisplatin for 24 h, under 2D and 3D culture conditions. c Cellular viability in response to cisplatin (60 μM for 72 h) of shp53 and ctrl MCF-7 cells under both culture conditions, by XTT assays. d–g MCF-7 cells treated with chloroquine (autophagy pharmacological inhibitor) and cisplatin, for 72 h, were used for d, e immunodetection of LC3-II and GAPDH and evaluation of cellular viability, by XTT assays, under 2D and 3D culture contexts (f, g). Densitometric analysis of p53 (a, b) and LC3-II (d, e) protein levels were normalized to the endogenous control (GAPDH). In all graphs, the results are presented as mean ± SEM from at least two independent experiments. Two-way analysis of variance and Bonferroni post hoc test were used for statistical analysis and the differences were considered significant for *P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001. ns highlights non-significant changes