Fig. 4: miR-21 regulates ASC pyroptosome formation.
From: miR-21 promotes NLRP3 inflammasome activation to mediate pyroptosis and endotoxic shock
a, b Immunofluorescence microscopy of LPS-primed macrophages untreated or stimulated with ATP and then stained for ASC and DNA (with DAPI). Scale bars: 20 μm. c Percentage of macrophages containing ASC foci. The quantification represents the mean of three independent experiments, with at least 50 cells counted in each experiment. d, e ASC oligomerization and redistribution assay in peritoneal macrophages treated as in a and b. Immunoblot analysis of ASC in cross-linked pellets (upper panels) and in cell lysates (lower panels)
