Fig. 2: miR-211 promoted DNA damage induced by carboplatin via inhibiting DDR genes. | Cell Death & Disease

Fig. 2: miR-211 promoted DNA damage induced by carboplatin via inhibiting DDR genes.

From: miR-211 facilitates platinum chemosensitivity by blocking the DNA damage response (DDR) in ovarian cancer

Fig. 2

a Carboplatin induced more significant DNA damage in ovarian cancer cells transfected with miR-211, whereas miR-211 inhibitor impaired the function of carboplatin as assessed by the comet assay. b Transfection of miR-211 increased the expression of γ-H2AX in HO8910 and SKOV3 cells treated with 0.25 mM carboplatin in the early stage, whereas amiR-211 inhibitor decreased γ-H2AX expression in these cells treated with 0.5 mM carboplatin. c Patients with higher levels of miR-211 exhibited an increased mutation frequency. d The dual luciferase assay confirmed that five DDR genes were targets of miR-211. e Transfection of miR-211 inhibited TDP1 expression in HO8910 and SKOV3 cells as assessed by western blot. f Suppression of miR-211 using inhibitor upregulated TDP1 expression in ovarian cancer cells

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