Fig. 1: Growth curve, immunophenotype, tetramer, and cell killing analysis of manufactured melanoma-associated antigen (MAGE)-A4 si-TCR gene-modified cells. | Cell Death & Disease

Fig. 1: Growth curve, immunophenotype, tetramer, and cell killing analysis of manufactured melanoma-associated antigen (MAGE)-A4 si-TCR gene-modified cells.

From: T-cell receptor gene therapy targeting melanoma-associated antigen-A4 by silencing of endogenous TCR inhibits tumor growth in mice and human

Fig. 1

a Transduction of si-TCR in human lymphocytes. Peripheral blood mononuclear cells from healthy donors were stimulated with anti-CD3 mAb and interleukin-2. Cells were cultured with or without retroviral transduction. Cell growth rate of si-TCR T cells and NGMC cells were monitored and the growth curve is shown. b Immunophenotype of si-TCR GMCs and NGMCs were detected by flow cytometric analysis and the histogram is shown. c Representative staining for gene-modified and unmodified cells with antibodies for cell surface markers. d GMCs and NGMCs were stained with MAGE-A4 tetramer and anti-CD8 mAb, percentage of tetramer positive cells among CD8+ cells is indicated. e Cell killing assay was performed in GMCs and NGMCs cocultured with KE4 and QG56 cell lines and the results were shown. All experiments were done at least from 3 donors’ PBMC and the representative data was shown. Repeated measures ANOVA was used for cell growth rate analysis and Mann–Whitney U-test was used for others. Data represent mean ± SEM, *P < 0.05, **P < 0.01

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