Fig. 3: Western blots of whole-cell lysates from the liver of WT and GsdmD−/− (KO) mice at 6 h after hemorrhagic shock with resuscitation (HS/R) or sham surgery (Sham), or 6 h after acetaminophen overdose (APAP) or controls. | Cell Death & Disease

Fig. 3: Western blots of whole-cell lysates from the liver of WT and GsdmD−/− (KO) mice at 6 h after hemorrhagic shock with resuscitation (HS/R) or sham surgery (Sham), or 6 h after acetaminophen overdose (APAP) or controls.

From: Gasdermin D protects against noninfectious liver injury by regulating apoptosis and necroptosis

Fig. 3: Western blots of whole-cell lysates from the liver of WT and GsdmD−/− (KO) mice at 6 h after hemorrhagic shock with resuscitation (HS/R) or sham surgery (Sham), or 6 h after acetaminophen overdose (APAP) or controls.

a, b Full-length (pro-)caspase-3 (Pro-cas3) and cleaved/active caspase-3 (Cl-cas3) levels. c, d Total RIPK1 (RIPK1) and MLKL (MLKL), and phosphorylated/active RIPK1 (p-RIPK1) and MLKL (p-MLKL) as markers of necroptosis. GAPDH or β-actin was used as loading controls. Images representative of results from at least three repeats. Each lane contains liver from one individual mouse. N = 4–5/group. Quantitation of band density was performed across at least three separate blots and mean ± SEM is shown in the graphs. NS = not significant. *p < 0.05, **p < 0.01, ***p < 0.001 compared with the indicated groups

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