Fig. 1: LS174T cells expressing XBP1(s) or ATF61–373 upregulate general UPR target genes. | Cell Death & Disease

Fig. 1: LS174T cells expressing XBP1(s) or ATF61–373 upregulate general UPR target genes.

From: Expression of UPR effector proteins ATF6 and XBP1 reduce colorectal cancer cell proliferation and stemness by activating PERK signaling

Fig. 1

a Quantitative RT-PCR analysis for XBP1(s) and downstream target genes upon induction of XBP1(s) expression. b Protein level of XBP1(s) and GRP78 in LS174T XBP1(s)Tet On cells. Cells were incubated for 0, 1 or 3 h with thapsigargin 200 nM. Note that transgenic XBP1(s) had a slightly lower molecular weight than endogenous XBP1(s). c Immunostaining of XBP1 in LS174T XBP1(s)Tet On cells. d Quantitative RT-PCR analysis for ATF61–373 and downstream target genes upon induction of ATF61–373 expression. e Protein level of GRP78 in LS174T ATF61–373 Tet On cells. Cells were treated with vehicle or doxycycline 1 μg/mL for 18 h. All data are shown as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001

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