Fig. 3: XBP1(s) or ATF61–373 expression upregulates PERK-eIF2α and inhibits global translation. | Cell Death & Disease

Fig. 3: XBP1(s) or ATF61–373 expression upregulates PERK-eIF2α and inhibits global translation.

From: Expression of UPR effector proteins ATF6 and XBP1 reduce colorectal cancer cell proliferation and stemness by activating PERK signaling

Fig. 3

a, c, e LS174T XBP1(s)Tet On cells. b, d, f LS174T ATF61–373 Tet On cells. a, b Protein levels of PERK and phosphorylated EIF2α. Cells were incubated for 0, 1 or 3 h with thapsigargin 200 nM. c, d Global protein translation rate, measured by 35SMethionine incorporation assay. e, f Percentage of apoptotic cells, measured with propidium iodide and Annexin V staining on Flow Cytometry. All data are shown as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001

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