Fig. 1: PP2A is required for myogenesis of C2C12 myoblasts.
From: Dephosphorylation of HDAC4 by PP2A-Bδ unravels a new role for the HDAC4/MEF2 axis in myoblast fusion
a Phase contrast images of C2C12 myoblasts treated (+OA) or not (no treatment) with 2 nM of okadaic acid. Pictures were taken at the indicated time points during differentiation and are representative of four experiments. b Phase contrast images of differentiating C2C12 myoblasts transfected with a control (siCTL) or a PP2Ac (siPP2Ac) siRNA. Pictures were taken at the indicated time points and are representative of three experiments. c RT-qPCR analysis of the indicated mRNA at day 0 (d0), day 1 (d1), day 3 (d3), and day 6 (d6) during differentiation of wild-type C2C12 myoblasts (n = 3, one-way anova, with Tukey’s post-hoc test, **P < 0.01, *P < 0.05). d Western blot analysis of I1PP2A and I2PP2A protein levels during differentiation of wild-type C2C12 cells, representative of two experiments. e, f RT-qPCR analysis e early (ID3 and Myogenin, n = 3) and f terminal (MLC2, n = 3 and MCK, n = 3) differentiation markers in differentiating C2C12 myoblasts transfected with a control (siCTL) or a PP2Ac (siPP2Ac) siRNA. Two-way anova, with Bonferroni correction, **P < 0.01, *P < 0.05. g Confocal imaging of differentiating C2C12 myoblasts transfected with a control (siCTL) or a PP2Ac (siPP2Ac) siRNA and stained for MHC expression (green), representative of three experiments. Nuclei (Hoechst) are in blue. h Fusion index at 6 days during differentiation (see methods for details) of C2C12 cells transfected with a control (siCTL) or a PP2Ac (siPP2Ac) siRNA (n = 4, unpaired t-test, two-tailed, ****P < 0.0001). Scale bars are 100 µm. Values are mean ± SD
