Fig. 2: PP2A-Bδ holoenzyme specifically controls myocyte fusion in vitro.
From: Dephosphorylation of HDAC4 by PP2A-Bδ unravels a new role for the HDAC4/MEF2 axis in myoblast fusion
a Confocal images of MHC (green) expression in wild-type C2C12 myoblasts or C2C12 myoblasts stably expressing a control (shCTR) shRNA or two shRNA against B55δ (shBδ), at day 6 during differentiation. Nuclei are stained with Hoechst (blue). Scale bars are 100 µm. Images are representative of three experiments. b, c Differentiation (b) and fusion (c) index (see methods for details) of wild-type C2C12 myoblasts or C2C12 myoblasts stably expressing a control shRNA (shCTR) or two shRNA against B55δ (shBδ). N = 3, one-way anova, with Tukey’s post hoc test. ***P < 0.001, ns: not significant. d Fusion index of control (shCTL) and Bδ-knocked down (shBδ) C2C12 myoblasts transfected with an empty vector (−) or a vector coding for rat PP2A-Bδ. N = 4, one-way anova with Tukey’s post-test, **P < 0.01, ****P < 0.0001
