Fig. 2: PCBP2 interacts with FMDV proteins. | Cell Death & Disease

Fig. 2: PCBP2 interacts with FMDV proteins.

From: Poly (rC) binding protein 2 interacts with VP0 and increases the replication of the foot-and-mouth disease virus

Fig. 2: PCBP2 interacts with FMDV proteins.

a, b The interaction between PCBP2 and FMDV proteins in mammalian overexpression systems was identified by coimmunoprecipitation experiment. The HEK293T cells (2 × 106) were transfected with HA-PCBP2 plasmid or expressing Flag-tagged VP2, 2B, 3A, L, VP0, 2C, 3C, and 3D proteins (5 μg each) for 24 h. Afterward, PCBP2 and FMDV proteins were analyzed by coimmunoprecipitation and immunoblotting analyses using the antibodies against flag and HA. c, d GST pull-down assay. Glutathione beads conjugated with GST or with GST-PCBP2 fusion protein were incubated with recombinant FMDV proteins. After washing three times, the proteins were eluted from the beads, and SDS-PAGE was performed. The presence of FMDV proteins was detected via immunoblotting utilizing the anti-flag monoclonal antibody. GST and GST-PCBP2 protein expression was confirmed via immunoblotting using the rabbit anti-GST polyclonal antibody. F flag tag, H HA tag, LC light chain

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