Fig. 1: The level of PGRN was significantly reduced in the kidney under diabetic conditions. | Cell Death & Disease

Fig. 1: The level of PGRN was significantly reduced in the kidney under diabetic conditions.

From: PGRN acts as a novel regulator of mitochondrial homeostasis by facilitating mitophagy and mitochondrial biogenesis to prevent podocyte injury in diabetic nephropathy

Fig. 1

a Representative photomicrographs and semiquantitation of PGRN immunohistochemical staining in human renal cortical tissue from normal subjects (n = 8), patients with diabetic nephropathy (DN) (n = 8), or diabetic patients without nephropathy (DM-NN) (n = 7). *P < 0.05 vs. Normal, #P < 0.05 vs. DM-NN. b Representative photomicrographs of PGRN immunohistochemical staining in the kidney from wild-type (WT) and PGRN-deficient (Grn−/−) mice (n = 6). Negative control by omission of the corresponding primary antibodies demonstrated no nonspecific staining. c Representative western blot gel documents and summarized data showing the relative protein levels of PGRN in human podocytes treated with high glucose (HG, final concentration 20 or 40 mmol/l in medium) for 48 h. *P < 0.05 vs. NG treatment. d Representative confocal microscopic images showing the expression of PGRN in podocytes of kidney from STZ-induced diabetic mice (n = 6), synaptopodin was used as a podocytes marker. The arrows indicate representative podocytes

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