Fig. 1: HIF1α is activated in VSMCs during Ang II-induced vascular remodeling.

WT mice were infused with saline or 1000 ng/kg/min Ang II for 28 days. a Immunofluorescence analysis of representative cross-sections of mice aortas for HIF1α (red) and α-SMA (green), nuclei was stained with DAPI. VSMCs were isolated form WT mice and treated with 1 μM Ang II for 24 h. b Hif1a mRNA was measured by qPCR analysis. c HIF1α protein was detected by western blot. **P < 0.01, ***P < 0.001, n = 3 per group, statistical significance was determined by the unpaired t-test