Fig. 5: Grb2 binds to Rad51 and PTEN.

a HeLa cells were exposed to 0.5 mM H₂O₂ for 2 h, and equal amounts of cell lysates were immunoprecipitated with the antibody to Grb2. b, c HEK293T or HeLa cells were exposed to 0.5 mM H₂O₂ for 2 h, and equal amounts of cell lysates were immunoprecipitated with the antibody of Grb2 or PTEN. d Equal amounts of HeLa cell lysates prepared were immunoprecipitated with the antibody of Grb2 or IgG. Samples were then blotted with indicated antibodies. e HEK293T cells and HeLa cells were stained with the Grb2 (green) and PTEN (red) antibodies following treatment with 0.5 mM H₂O₂ for 2 h. Similar experiments were repeated at least three times. f HeLa cells were exposed to 0.5 mM H₂O₂ for 2 h, and equal amounts of cell lysates were immunoprecipitated with the antibody of Grb2 or Rad51. g HeLa cells were transfected with the plasmids expressing PTEN mutants for 36 h. Equal amounts of cell lysates were prepared and immunoprecipitated with the antibody to Grb2. The ratios of PTEN in the immunoprecipitated group to GRB2 in the immunoprecipitated group were presented under the blots. h The total homogenate (TH), cytoplasm (Cyto), nuclear fraction (Nu) or nuclear precipitation fraction (Nup) of HeLa cells were extracted. Equal amounts of samples were immunoprecipitated with the antibody of Grb2. Similar experiments were repeated at least three times