Fig. 6: Depletion of Grb2 prevents PTEN from translocating into nucleus. | Cell Death & Disease

Fig. 6: Depletion of Grb2 prevents PTEN from translocating into nucleus.

From: Grb2 binds to PTEN and regulates its nuclear translocation to maintain the genomic stability in DNA damage response

Fig. 6: Depletion of Grb2 prevents PTEN from translocating into nucleus.

a After treatment with 0.5 mM H2O2, TH, Cyto, Nu, and the Nup were extracted from HeLa cells and analyzed by immunoblotting with the antibodies indicated. b After 48 h transfection with the control (Mock) or Grb2 siRNA (siGrb2), HeLa cells were exposed to 0.5 mM H2O2 for up to 2 h, and then TH, and Nu were extracted and analyzed by immunoblotting with the antibodies indicated (Nu: soluble plus insoluble Nu). c, d HeLa cells were transfected with the control (Mock) or Grb2 siRNA (siGrb2) for 48 h, and treated with 0.5 mM H2O2 (C: 1 h; D: 2 h) before immunofluorescence analysis of PTEN localization. Similar experiments were repeated at least three times

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