Fig. 6: 48 h aaD in primary adult rat hepatocytes increases Drosha protein stability.
From: Amino acid-induced regulation of hepatocyte growth: possible role of Drosha
a Western blot analysis and b quantification of Drosha protein level in primary adult rat hepatocytes cultured in full-aa medium (DMEM) or in absence of aa (w/o aa); c Western blot analysis and d quantification of Drosha protein level in primary adult rat hepatocytes cultured in full-aa medium (DMEM) or in medium without aa supplemented with essential aa (w/o aa+e.aa). The protein content of cells isolated from the same liver was analyzed just before treatment (t0) or after 1 h, 2 h, and 8 h from the addition of cycloheximide (CHX) (50 µm). Values are means ± SEM (n = 3). *p < 0.05, ***p < 0.0005
