Fig. 5: Effects of NEDD4-2 knockdown on surface KCNQ and GluA1 expression, GluA1-NEDD4-2 bindings, GluA1 ubiquitination, and neuronal activity in response to AMPA in PLPP/CIN^−/− mice.

a–c Effects of NEDD4-2 knockdown on surface KCNQ and GluA1 expression following KA injection in PLPP/CIN^−/− mice. As compared to control siRNA, NEDD4-2 siRNA increases surface GluA1, but not KCNQ2/3/5, expression in PLPP/CIN^−/− mice under physiological condition and after KA injection. a Representative western blots of surface and total KCNQ and GluA1 expressions. b, c Quantification of surface and total KCNQ and GluA1 expressions based on western blot data. Open circles indicate each individual value. Horizontal bars indicate mean value (mean ± S.E.M.; *^,#p < 0.05 vs. saline- and control siRNA-treated animals, respectively; n = 7). d, e Effects of NEDD4-2 siRNA on GluA1 ubiquitination in PLPP/CIN^−/− mice. NEDD4-2 knockdown reduces GluA1-NEDD4-2 bindings and GluA1 ubiquitination in PLPP/CIN^−/− mice under physiological condition and after KA injection. d Representative western blots of GluA1 ubiquitination. e Quantification of GluA1 ubiquitination based on western blot data. Open circles indicate each individual value. Horizontal bars indicate mean value (mean ± S.E.M.; *^,#p < 0.05 vs. saline- and control siRNA-treated animals, respectively; n = 7). f, g Effects of NEDD4-2 siRNA on neuronal activity in response to AMPA in PLPP/CIN^−/− mice. NEDD4-2 knockdown increases the neuronal activity in response to AMPA in PLPP/CIN^−/− mice. f Representative EEG traces and frequency-power spectral temporal maps in response to AMPA. g Quantification of total EEG power in response to AMPA (mean ± S.E.M.; n = 7, respectively)