Fig. 2: TCTP downregulation resulted in cardiomyocyte death. | Cell Death & Disease

Fig. 2: TCTP downregulation resulted in cardiomyocyte death.

From: Translationally controlled tumor protein (TCTP) plays a pivotal role in cardiomyocyte survival through a Bnip3-dependent mechanism

Fig. 2

a–d NRVMs were transfected with various doses of TCTP siRNA #1 or siRNA #2 for 72 h. e, f NRVMs were treated with various concentrations of DHA for 48 h. a, c, e Protein expression of TCTP and GAPDH was analyzed after TCTP siRNAs transfection or DHA treatment (n = 4). b, d, f Cell death was determined by calcein-AM/ethidium homodimer-1 staining (n = 4). Live and dead cells were distinguished by calcein-AM (green) and ethidium homodimer-1 (red) staining, respectively. Scale bar, 200 μm. *P < 0.05, **P < 0.01, ***P < 0.001 vs 0. One-way ANOVA followed by Tukey’s test

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