Fig. 2: Conditioned medium from C-F5M2 cells promoted F5M2 cell migration and invasion in transwell assays (magnification ×200). | Cell Death & Disease

Fig. 2: Conditioned medium from C-F5M2 cells promoted F5M2 cell migration and invasion in transwell assays (magnification ×200).

From: Self-seeding circulating tumor cells promote the proliferation and metastasis of human osteosarcoma by upregulating interleukin-8

Fig. 2

a Representative images of F5M2 cells subjected to the transwell migration assay with conditioned medium from F5M2 cells loaded in the lower chamber. b Representative images of F5M2 cells subjected to the transwell migration assay with conditioned medium from C-F5M2 cells loaded in the lower chamber. c Representative images of C-F5M2 cells subjected to the transwell migration assay with conditioned medium from F5M2 cells loaded in the lower chamber. d Representative images of C-F5M2 cells subjected to the transwell migration assay with conditioned medium from C-F5M2 cells loaded in the lower chamber. e Representative images of F5M2 cells subjected to the transwell invasion assay with conditioned medium from F5M2 cells loaded in the lower chamber. f Representative images of F5M2 cells subjected to the transwell invasion assay with conditioned medium from C-F5M2 cells loaded in the lower chamber. g Representative images of C-F5M2 cells subjected to the transwell invasion assay with conditioned medium from F5M2 cells loaded in the lower chamber. h Representative images of C-F5M2 cells subjected to the transwell invasion assay with conditioned medium from C-F5M2 cells loaded in the lower chamber. i Conditioned medium from C-F5M2 cells promoted the migration of F5M2 cells in the transwell assays (P < 0.01). j Conditioned medium from C-F5M2 cells promoted the invasion of F5M2 cells in the transwell assays (P < 0.01). *P < 0.05, **P < 0.01, ***P < 0.001

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