Fig. 1: CXCL12 loaded gel (CLG) attracted CXCR4 positive cells.

A Migration of B16-hCXCR4-GFP cells Cell Tracker Green toward CXCL12 in culture medium or CLG (300 ng/ml) with AMD3100 (10–100 nM) or anti-CXCR7 (10 µg/ml). The results are expressed as index (B16-hCXCR4-GFP cells migrated toward CXCL12 or CLG/ B16-hCXCR4-GFP cells migrated toward BSA (0.5% BSA in culture medium) or EG). Each column represents the mean ± S.D. (n = 3). Statistical significances were calculated by Mann-Whitney U test. **p < 0.01 CXCL12 vs BSA or ***p < 0.001 CLG vs EG; °p < 0.05; °°°p < 0.001 AMD3100 vs CXCL12 or AMD31000 vs CLG. B Migration assay of B16-hCXCR4-GFP and B16-GFP cells. B16-hCXCR4-GFP cells and B16-GFP were exposed to the chemokine CXCL12 in culture medium or CLG (300 ng/ml) with AMD3100 (100 nM) in a transwell culture plate. The results are expressed as index. Each column represents the mean ± S.D. (n = 3). Statistical significances were calculated by Student’s t-test. In B16-hCXCR4-GFP cells ***p < 0.001 CXCL12 vs BSA or CLG vs EG; °°°p < 0.001 AMD3100 vs CXCL12 or AMD31000 vs CLG; in B16-GFP *p < 0.05 CXCL12 vs BSA or *p < 0.05 CLG vs EG; °p < 0.05 AMD3100 vs CXCL12 or °°p < 0.01 AMD31000 vs CLG. C Long term migration of B16-hCXCR4-GFP cells Cell Tracker Green toward CLG (300 ng/ml). The results are expressed as B16-hCXCR4-GFP migrated toward CLG/ B16-hCXCR4-GFP migrated toward EG for 3, 7, 14, 21, 28 days. Each column represents the mean ± S.D. (n = 3). Statistical analysis of the difference between cells migrated toward CLG and EG at same time point were calculated by Student’s t-test. ***p < 0.001; **p < 0.01; *p < 0.05 CLG vs EG