Fig. 5: Mitophagy was induced by fluoxetine in primary astrocytes.

a Immunofluorescence for colocalization of LC3 and mitochondria in primary astrocytes. Cells were treated with 10 μM fluoxetine whose mitochondria were labeled by MitoTracker Deep Red. Each green spot represents one autophagosome. Scale bar: 20 μm. b Pearson’s correlation coefficient for colocalization of LC3 and mitochondria. c Immunofluorescence for colocalization of mitochondria (MitoTracker Green) and lysosomes (LysoTracker Red) in primary astrocytes. Scale bar: 60 μm. d Pearson’s correlation coefficient for colocalization of lysosomes and mitochondria. e–h Western blotting and quantitative analysis of Parkin and TOMM20 expressed in cytoplasm and mitochondria. Data are representative of four independent experiments and are expressed as mean ± SD. *p < 0.05 vs. Con group, ^#p < 0.05, ^##p < 0.01 vs. Con group^{, $}p < 0.05, ^$$p < 0.01 vs. CORT group