Fig. 2: Cdk5 interacts with Fbxw7. | Cell Death & Disease

Fig. 2: Cdk5 interacts with Fbxw7.

From: Site-specific phosphorylation of Fbxw7 by Cdk5/p25 and its resulting decreased stability are linked to glutamate-induced excitotoxicity

Fig. 2

a Following transfection of HEK293 cells with the indicated combination of vectors, cell lysates were subjected to IP using Flag-conjugated beads. Immunoprecipitates and WCL were resolved by SDS-PAGE and analyzed by IB analysis. b HEK293 cells were transfected with Flag-Fbxw7 alone or in combination with either V5-Cdk5 WT/Myc-p25 or V5-Cdk5 DN/Myc-p25. IP followed by IB was performed as described above. c Primary cultures of cortical neurons were maintained until days in vitro(DIV) 11. IP was performed with anti-Fbxw7 or anti-Cdk5 antibodies. Rabbit anti-IgG and mouse anti-IgG antibodies were used as IP controls. Fbxw7 was detected by IB after IP pulldown with anti-Cdk5 antibody and vice versa

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