Fig. 6: Inverse expression patterns of Fbxw7 and its substrate c-Jun detected in cortical neurons during glutamate excitotoxicity.

a Primary cultures of cortical neurons were exposed to 200 μM glutamate for the indicated time periods or b cultures were exposed for 2 h to 200 μM glutamate in the presence or absence of 10 μM MG132. a, b Cell lysates were harvested and analyzed by IB. c Primary cultures of cortical neurons were infected with lentiviral particles containing shRNA against either Cdk5 or control (Scramble) at DIV3 and subsequently maintained until DIV 11. Cultures were then exposed to 200 μM glutamate for the indicated time periods. Cell lysates were subjected to IB analysis. d After normalization against GAPDH, relative levels of Fbxw7 at the designated time periods were evaluated against time point 0 and are shown in the graph. Each point represents the mean ± SD from five independent experiments. ^*p < 0.05; ^***p < 0.001^. e Primary cultures of cortical neurons were exposed to 200 μM glutamate for up to 6 h. Cell lysates were harvested and analyzed by IB. f After normalization against GAPDH, relative fold intensity of Fbxw7 and c-Jun signal was quantified over the control at time 0 (value = 1). Each point represents the mean ± SD from three independent experiments. ^**p < 0.01; ^***p < 0.001