Fig. 2: Effect of cP1P on glucose metabolism in UCB-MSCs. | Cell Death & Disease

Fig. 2: Effect of cP1P on glucose metabolism in UCB-MSCs.

From: O-cyclic phytosphingosine-1-phosphate stimulates HIF1α-dependent glycolytic reprogramming to enhance the therapeutic potential of mesenchymal stem cells

Fig. 2

a, b UCB-MSCs were incubated with cP1P (1 μM) for 24 h. a The mRNA expression levels of glucose metabolism-associated genes were assessed by RT2 Profiler PCR array, heat maps with hierarchical clustering shown in left panel were acquired by using the GeneGlobe Data Analysis Center on Qiagen website (http://www.qiagen.com/kr/shop/genes-and-pathways/data-analysis-center-overview-page/). n = 3. b Hexokinase activity was measured by commercial kit. n = 6. c UCB-MSCs were pretreated with cP1P (1 μM) for 30 min prior to normoxia or hypoxia incubation for 48 h. Intracellular lactate levels in UCB-MSCs were measured by commercial kit. n = 4. d, e UCB-MSCs were incubated with cP1P (1 μM) for 24 h. d OCR changes under mitochondrial stress test were assessed by Seahorse XF24 Extracellular Flux Analyzer. Quantitative data of basal respiration, maximal respiration, proton leak, ATP production, and spare respiratory capacity are shown in bottom panel. n = 4. e ECAR changes under glycolysis stress test were assessed by Seahorse XF24 Extracellular Flux Analyzer. Quantitative data of glycolysis, glycolytic capacity, and glycolytic reserve are shown in bottom panel. n = 4. f NHE1 mRNA expressions in UCB-MSCs treated with cP1P (1 μM) for 0, 6, or 12 h. n = 4. Gene expression levels were normalized by 18 S rRNA expression levels. g UCB-MSCs were incubated with cP1P (1 μM) for 24 h. Intracellular alkalization was measured by using BCECF-AM staining. n = 6. Quantitative data are presented as a mean ± S.E.M with scatter plots. * indicates p < 0.05

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